Determining the subclass of a rat antibody is important when selecting the best purification method. IgG2a, IgG2b, and IgG2c can be purified with Protein A columns at a pH of 7-8. IgG1 isotypes bind best to Protein A at a slightly higher pH of 8-9.
The Iso-Gold™ Rapid Rat Antibody Isotyping Kit is a very easy-to-use 5-minute dipstick assay with ELISA-like sensitivity for determining rat antibody subclass. The assay can be run on both tissue culture supernatant fluid and on rat ascites fluid. Protocols are provided for both sample types.
- Rat IgG1, IgG2a, IgG2b , and IgG2c tests, 10 dipsticks
- Sample Diluent Buffer, 4 mL
Storage Temperature: Store Sample Diluent Buffer refrigerated (4-8°C). Dipsticks can be stored at room temperature or 4-8°C
Shipping Temperature: Refrigerated (2-8°C)
Stability: Two years from date of manufacture.