Determining the class and subclass of a monoclonal antibody is important when selecting the best purification method. IgA and IgM are often best purified by size (gel exclusion) or affinity columns, whereas IgG2a and IgG2b can be purified with Protein A columns at a pH of 7-8. IgG1 monoclonals bind best to Protein A at a slightly higher pH of 8-9.
The Iso-Gold™ Rapid Mouse-Monoclonal Isotyping Kit is a 5 minute lateral flow assay with ELISA sensitivity for determining mouse monoclonal antibody class and subclass. The assay can be run on both tissue culture supernatant fluid and on mouse ascites fluid. Protocols are provided for both sample types.
- IgG1, IgG2a, and IgG2b test, 5 cassettes
- IgG3, IgA, and IgM test, 5 cassettes
- Sample Diluent Buffer, 25 mL
Storage Temperature: Store Sample Diluent Buffer refrigerated (2-8°C). Cassettes can be stored at room temperature.
Shipping Temperature: Refrigerated (2-8°C)
Stability: 2 years from date of manufacture