Iso-Gold^™ Rapid Rat Antibody Isotyping Kit, 10 tests

Description

Determining the subclass of a rat antibody is important when selecting the best purification method. IgG_2a, IgG_2b, and IgG_2c can be purified with Protein A columns at a pH of 7-8. IgG1 isotypes bind best to Protein A at a slightly higher pH of 8-9.

The Iso-Gold™ Rapid Rat Antibody Isotyping Kit is a very easy-to-use 5-minute dipstick assay with ELISA-like sensitivity for determining rat antibody subclass. The assay can be run on both tissue culture supernatant fluid and on rat ascites fluid. Protocols are provided for both sample types.

Specifications:

Kit Contents:

• Rat IgG₁, IgG_2a, IgG_2b , and IgG_2c tests, 10 dipsticks
• Sample Diluent Buffer, 4 mL

Storage Temperature: Store Sample Diluent Buffer refrigerated (4-8°C). Dipsticks can be stored at room temperature or 4-8°C

Shipping Temperature: Refrigerated (2-8°C)

Stability: Two years from date of manufacture.