Rat Rena ELISA Kit, 1 plate

$565.40

Rating: SKU: R-RENA-E-001. Category: Tags: , ,

The Rat Rena ELISA kit is a “Research Use Only” ELISA for the quantitative detection of Kim-1 in rat urine.

Product Description

Kit is being discontinued. Limited quantities available. Please contact us at sales@bioassayworks.com for more information.

Kim-1 is a type I trans-membrane structural glycoprotein located in the renal proximal tubule epithelial cells.  These cells undergo regeneration after various forms of injury and shed Kim-1 antigen into the urine.  Thus, urinary Kim-1 is an early and specific biomarker for tubular kidney injury. Kim-1 has become widely recognized by many organizations and agencies, including the FDA, as an excellent biomarker in pre-clinical studies to monitor acute kidney tubular toxicity by identifying adverse reactive drugs and therapeutic agents in drug development.

Kidney injury caused by therapeutic agents and drug induction is a common type of injury requiring appropriate monitoring and intervention.  Current standards using blood urea nitrogen and creatinine are considered late indicators of kidney injury and are often non-specific. Kim-1 has been shown continually to outperform traditional biomarkers of kidney injury in preclinical biomarker studies. The detection of Kim-1 can occur in as little as six hours post injection of an agent known to cause kidney injury.

The Rat Rena ELISA Kit is a quantitative test for the detection of Kim-1 in rat urine samples.

Specifications:

Kit Contents:

  • Rat Kim-1 antibody coated plate, 12 x 8 well microwell strips coated with anti-rat Kim-1 antibody in a resealable pouch with desiccant.
  • Rat Kim-1 Calibrator, Two vials containing lyophilized recombinant rat Kim-1 in a buffered protein based stabilizer – 40 ng/mL after reconstitution.
  • Reconstitution Solution, 1.8 mL of laboratory grade water with preservative.
  • Kim-1 Negative Control, 200µL of a buffered protein solution.
  • Sample Dilution Buffer, 12 mL of phosphate buffered saline, protein stabilizer, detergent, preservative and yellow dye.
  • Rat Kim-1 MAb Solution, 6 mL of anti-Rat Kim-1 monoclonal antibody conjugated to biotin in phosphate buffered saline, protein stabilizer, detergent, preservative and blue dye.
  • Peroxidase Conjugate Solution, 12 mL of horseradish peroxidase conjugated streptavidin in a buffered protein solution, enzyme stabilizers and preservative.
  • 20x Wash Solution, 50 mL of 20x phosphate buffered saline, detergent and preservative.
  • ABTS Substrate Solution, 12 mL ABTS Substrate Solution
  • Stop Solution, 12 mL of 1% Lauryl Sulfate with preservative. Store at room temperature.

Storage Temperature: Refrigerated (2-8° C)

Shipping Temperature: Refrigerated (2-8° C)

Stability: Stable up to the expiration date printed on the kit label.

Product is for research use only.

Additional information

Weight 4 lbs
Dimensions 8 x 8 x 8 in

Documents

Product Flyer – Assays for KIM-1 Acute Kidney Injury Biomarker

Product Insert – L-670-03

SDS – L-SDS-672-01

References

  1. Se Won Oh, Eun Seong Lee, Sejoong Kim, Ki Young Na, Dong Wan Chae, Suhnggwon Kim and Ho Jun Chin. Bilirubin attenuates the renal tubular injury by inhibition of oxidative stress and apoptosis. BMC Nephrology. 2013; 14:105.