The Latex-in-a-Box™ Conjugation Kit contains the reagents required to prepare highly-reactive antibody or soluble protein latex-particle conjugates that can be utilized in lateral-flow assays.
The key to latex nanoparticle lateral-flow assays is the ability to covalently attach antibodies/proteins to intensely colored latex nanoparticles. The Latex-in-a-Box™ Conjugation Kit enables optimal binding of an antibody/protein to RED 0.3 micron latex nanoparticles while retaining a high degree of specific activity through adjusting the pH of the latex suspension to slightly above the isoelectric point of the coating antibody/protein. This is done through a series of pH titrations with the provided buffers. The Latex-in-a-Box™ kit allows researchers and assay developers to quickly (in less than 1 hour) determine the pI and optimal coating conditions for your antibody or soluble protein and to perform the conjugation reaction. Both passive coating procedures and covalent coupling procedures are provided.
Reagents are also provided to coat the resultant latex conjugates on polyester of glass fiber ribbon.
- LP02-O007 – 2.5% RED 0.3 micron latex particles, 7 mL
- BUFA-001 – Buffer Solution A, 1.0 mL
- BUFB-001 – Buffer Solution B, 1.0 mL
- BUFC-001 – Buffer Solution C, 1.0 mL
- BUFD-001 – Buffer Solution D, 1.0 mL
- BLK-002 – BSA Blocking Stabilizer Solution, 2.0 mL
- CDB-002 – Latex Conjugate Drying Buffer, 2.0 mL
Storage Temperature: Refrigerated (2-8°C), do not freeze
Shipping Temperature: Ambient temperatures
Stability: 2 years from date of manufacture