Iso-Gold™ Rapid Mouse-Monoclonal Isotyping Kit, 10 tests

$203.10

Rating: SKU: ISOT02-010. Category: Tags: , ,

The Iso-Gold™ Mouse Monoclonal Isotyping Kit determines the mAb class and subclass in a rapid 5-minute lateral flow assay. Both tissue culture supernatant fluid and mouse ascites fluid can be tested.

Product Description

Determining the class and subclass of a monoclonal antibody is important when selecting the best purification method. IgA and IgM are often best purified by size (gel exclusion) or affinity columns, whereas IgG2a and IgG2b can be purified with Protein A columns at a pH of 7-8. IgG1 monoclonals bind best to Protein A at a slightly higher pH of 8-9.

The Iso-Gold™ Rapid Mouse-Monoclonal Isotyping Kit is a 5 minute lateral flow assay with ELISA sensitivity for determining mouse monoclonal antibody class and subclass. The assay can be run on both tissue culture supernatant fluid and on mouse ascites fluid. Protocols are provided for both sample types.

Specifications:

Kit Contents:

  • IgG1, IgG2a, and IgG2b test, 10 cassettes
  • IgG3, IgA, and IgM test, 10 cassettes
  • Sample Diluent Buffer, 45 mL

Storage Temperature: Store Sample Diluent Buffer refrigerated (2-8°C). Cassettes can be stored at room temperature.

Shipping Temperature: Refrigerated (2-8°C)

Stability: 2 years from date of manufacture

Additional information

Weight .6 lbs
Dimensions 7 x 4.5 x 3.5 in

Documents

Product Insert – L-PI-ISOT02-010-01

SDS – L-SDS-102-02

References

  1. Hongxia Shao, Jianqiang Ye, Amy L. Vincent, Nicole Edworthy, Andrea Ferrero, Aijian Qin, Daniel R. Perez. A novel monoclonal antibody effective against lethal challenge with swine-lineage and 2009 pandemic H1N1 influenza viruses in mice. Virology. 2011; 417(2):379-384.
  2. Ki Joon Cho, Kwang W. Hong, Se-Ho Kim, Jong Hyeon Seok, Sella Kim, Ji-Hye Lee, Xavier Saelens, Kyung Hyun Kim. Insight into Highly Conserved H1 Subtype-Specific Epitopes in Influenza Virus Hemagglutinin. PloS one. 2014; 9(2).