Human Rena ELISA Kit, 1 plate

$620.20

Rating: SKU: H-RENA-E-001. Category: Tags: , ,

The Human Rena ELISA kit is a “Research Use Only” ELISA for the quantitative detection of KIM-1 in human urine.

Product Description

Kit is being discontinued. Limited quantities available. Please contact us at sales@bioassayworks.com for more information.

KIM-1 is a type I trans-membrane structural glycoprotein located in the renal proximal tubule epithelial cells.  These cells undergo regeneration after various forms of injury and shed KIM-1 antigen into the urine.  Thus, urinary KIM-1 is an early and specific biomarker for tubular kidney injury.  KIM-1 has become widely recognized by many organizations and agencies, including the FDA, as an excellent biomarker in pre-clinical studies to monitor acute kidney tubular toxicity by identifying adverse reactive drugs and therapeutic agents in drug development.

Kidney injury caused by therapeutic agents and drug induction is a common type of injury requiring appropriate monitoring and intervention.  Current standards using blood urea nitrogen and creatinine are considered late indicators of kidney injury and are often non-specific.  KIM-1 has been shown continually to outperform traditional biomarkers of kidney injury in preclinical biomarker studies. The detection of KIM-1 can occur in as little as six hours post injection of an agent known to cause kidney injury.

The Human Rena ELISA Kit is a quantitative test for the detection of KIM-1 in human urine samples.

Specifications:

Kit Contents:

  • Human KIM-1 antibody coated plate, 12 x 8 well microwell strips coated with anti-human KIM-1 antibody in a resealable pouch with desiccant.
  • Human KIM-1 Calibrator, Two vials containing lyophilized recombinant Human KIM-1 in a buffered protein based stabilizer – 40 ng/mL after reconstitution.
  • Reconstitution Solution, 1.8 mL of laboratory grade water with preservative.
  • KIM-1 Negative Control, 200µL of a buffered protein solution.
  • Sample Dilution Buffer, 12 mL of phosphate buffered saline, protein stabilizer, detergent, preservative and yellow dye.
  • Human KIM-1 MAb Solution, 6 mL of anti-Human KIM-1 monoclonal antibody blend in phosphate buffered saline, protein stabilizer, detergent, preservative and blue dye.
  • Peroxidase anti-Human KIM-1, 12 mL of horseradish peroxidase labeled anti-Mouse IgG in a buffered protein solution, enzyme stabilizers and preservative.
  • 20x Wash Solution, 50 mL of 20x phosphate buffered saline, detergent and preservative.
  • ABTS Substrate Solution, 12 mL ABTS Substrate Solution
  • Stop Solution, 12 mL of 1% Lauryl Sulfate with preservative. Store at room temperature.

Storage Temperature: Refrigerated (2-8° C)

Shipping Temperature: Refrigerated (2-8° C)

Stability: Stable up to the expiration date printed on the kit label.

Product is for research use only.

Additional information

Weight 4 lbs
Dimensions 8 x 8 x 8 in

Documents

Product Flyer – Assays for KIM-1 Acute Kidney Injury Biomarker

Product Insert – L-650-05

SDS – L-SDS-652-01

References

  1. Alexandra JM Zwiers, Saskia N de Wildt, Joost van Rosmalen, Yolanda B de Rijke, Erik AB Buijs, Dick Tibboel and Karlien Cransberg. Urinary neutrophil gelatinase-associated lipocalin identifies critically ill young children with acute kidney injury following intensive care admission: a prospective cohort study. Critical Care. 2015; 19:181.
  2. Flavio Ribichini, Alessia Gambaro, Michele Pighi, Gabriele Pesarini, Pietro Manuel Ferraro, Cecilia Zuppi, Silvia Baroni, Romina Penitente, Valeria Ferrero, Corrado Vassanelli. Effects of prednisone on biomarkers of tubular damage induced by radiocontrast in interventional cardiology. JNEPHROL 2013; 26( 3 ): 586-593.
  3. Lyubov Chaykovska, Fabian Heunisch, Gina von Einem, Markus L. Alter, Carl-Friedrich Hocher, Oleg Tsuprykov, Thomas Dschietzig, Axel Kretschmer, Berthold Hocher. Urinary Vitamin D Binding Protein and KIM-1 Are Potent New Biomarkers of Major Adverse Renal Events in Patients Undergoing Coronary Angiography. PloS one. 2016; 11(1): e0145723.